Objectives: We aimed to investigate the risk factors for bladder calculi in patient with spinal cord injury. Study Design: Prospective study. Methods: From January 2019 to June 2020 in our hospital spinal cord injury rehabilitation center, 41 patients with bladder stones and surgical treatment of spinal cord injury were found. Multivariate analysis was performed to determine the risk factors of initial bladder stones. Results: Among the 41 patients, 38（82.9%）were male, 22 (53.7%) were with high spinal cord injury (T6 or above), including 14 cases of cervical injury, 4 cases (9.8%) of Cauda Equina injury, and 15 cases (34.1%) of other thoracolumbar injuries. The main cause of stone formation was indwelling catheterization. There were 22 cases, accounting for 53.7%. Involuntary leakage and intermittent catheterization within 3 times a day were also easy to cause stones, accounting for 10 cases (24.4%) and 6 cases (14.6%) respectively. Only 3 cases (7.3%) had intermittent catheterization 3 to 6 times a day. Conclusions: Bladder calculi were most likely to develop within one year of spinal cord injury, indewelling catheterization is the most common cause. In order to prevent the formation of bladder stone, early removal of urethral catheter is recommended and indwelling catheters should be avoided if possible in patient with spinal cord injury.